Extended Data Fig. 5: Metabolic profiles of chemical perturbations in A549 cancer cells.
From: Combining CRISPRi and metabolomics for functional annotation of compound libraries
A) Dynamic monitoring of cell confluence (% well area covered by cells) in A549 cancer cell line in the presence of sgRNAs targeting three different sections of thymidylate synthase, in addition to a control sgRNA inducing cell death (lethal control, gLethal) by targeting multiple sections in the genome. B) Dynamic monitoring of cell confluence in A549 cancer cell line treated with different drugs. C) The volcano plots illustrate metabolic changes (that is log2 fold-change and p-value significance) for 1593 putatively annotated metabolites in A549 cells 48 hours after treatment with 14 different drugs. Pvalues are estimated by two-sided t-test analysis. 3 drugs are targeting TYMS (methotrexate, pemetrexed and 5-fluorouracil). The remaining drugs act on largely different cellular processes, such as central metabolism (oxamate, dichloroacetate, carbonyl cyanide, 2-deoxyglucose, UK5099), nucleotide biosynthesis (mercaptopurine), mevalonate pathway (atorvastatin), DNA repair (camptothecin, cisplatin, doxorubicin) and dopamine receptors (trifluoperazine).
