Extended Data Fig. 6
From: Biosynthesis of cyanobacterin, a paradigm for furanolide core structure assembly
In vitro assay of At4CL1. A) HPLC analysis of in vitro assay of At4CL1 which successfully converted 4-coumaric acid (black) to 4-coumaroyl-CoA (blue). Conversion was unsuccessful in the negative control (minus enzyme; blue). B) HR-LCMS analysis of in vitro conversion of 4-coumaric acid to 4-coumaroyl-CoA. Presence of 4-coumaroyl-CoA is confirmed by MS1 (top) and MS2 (bottom).
