Extended Data Fig. 7: 1H-NMR analysis of 1,4-hydride shift experiments enabled by one-pot enzymatic synthesis in D2O.
From: Biosynthesis of cyanobacterin, a paradigm for furanolide core structure assembly
Compared to the control performed in H2O (top), the experiment in D2O lead to deuterium incorporation at three positions, at two of which by keto/enol-tautomerism (blue and green dots) and one by 1,4-deuterium-shift (yellow).
