Extended Data Fig. 5: Recording memory of antigen encounter.
From: Scalable biological signal recording in mammalian cells using Cas12a base editors
a, Constructs used for SynNotch-induced guide expression. b, Cellular memory of antigen encounter via SynNotch, in GFP* HEK cells transfected with guide, SynNotch, and base editor, then cocultured with -/+CD19 HEK cells. Data shown for 3 independent replicates c, Constructs used for recording CD19-CAR activation in Jurkat cells. The top two constructs were stably integrated, and the bottom two were electroporated. d, Representative flow cytometry histograms for signal recording of CD19 antigen encounter in Jurkat cells. Histograms show BFP (induced by NFAT promoter) vs GFP (from successful base editing event of GFP* reporter) in engineered Jurkat CAR-T cells, after 48 hours of co-culture with K562 cells -/+CD19. To go with Fig. 2d.
