Extended Data Fig. 6: Characterization of recording parameters.
From: Scalable biological signal recording in mammalian cells using Cas12a base editors
a, GFP* HEK cells stably transduced with lentiviral NFKBp-guide and transfected with base editor were stimulated with 100 ng/mL TNFα for 3 days, then analyzed via flow cytometry. Randomly selected sub-populations of cells of the indicated size were analyzed using downsampling. Statistical analysis was performed using a two-sided t test without adjustment for multiple comparisons. Data shown for 3 independent replicates. To go with Fig. 3a. b, The mean GFP fluorescence for each downsampled cell population for the NFKB-recording circuit, +TNFα condition is plotted against the number of cells analyzed, to go with Fig. 3a. Dotted lines with gray shading indicate standard deviation of 3 independent replicates. c, Dose response for GFP* HEK cells stably transduced with lentiviral NFKBp-guide and transfected with base editor. Mean BFP fluorescence is shown 3 days post-transfection and stimulation with TNFα. Error bars indicate standard deviation of 3 independent replicates. To go with Fig. 3b. d, Mean BFP (expressed under NFKB promoter) plotted against mean GFP (“memory” reporter) for TNFα dose-response experiment. Data were fitted using a linear regression. Error bars indicate standard deviation of 3 independent replicates. e, Experimental timeline used for testing different durations of TNFα stimulation.
