Extended Data Fig. 7: Recording history of NFKB signaling at the AAVS1 locus.
From: Scalable biological signal recording in mammalian cells using Cas12a base editors
a, Left, constructs used for signal recording using inducible AAVS1 targeting guides in HEK cells. Right, experimental timeline used for genomic DNA (gDNA) collection. b, Single-input recording at the AAVS1 locus in HEK cells transfected with base editor and NFKB-driven crAAVS1_4, and stimulated with TNFα for 3 days. The %A→G conversion as measured by MiSeq is shown for A11 (left) and A14 (right), with number indicating position of A relative to PAM. Data shown for 3 independent replicates. c, Left, constructs used for recording at the AAVS1 locus in Jurkat cells. Right, experimental timeline. d, Recording of NFKB-mediated inflammation in Jurkat cells at the AAVS1 locus, as measured by MiSeq. Data shown for 3 independent replicates.
