Extended Data Fig. 1: Base editing with hyperCas12a for signal recording.
From: Scalable biological signal recording in mammalian cells using Cas12a base editors
a, Left, representative flow cytometry histograms showing GFP fluorescence in HEK cells 3 days post-transfection with GFP* reporter, U6-driven guide and wild type (WT) or mutant (hyper) dCas12a-ABE. crLacZ, non-targeting control. Right, quantification of percentage GFP + cells for 3 independent replicates. To go with Fig. 1c. b, Representative flow cytometry histograms for GFP* HEK cells transfected with the dox-recording constructs, 3 days post-transfection and stimulation with dox. To go with Fig. 1f. c, Representative flow cytometry histograms for GFP* HEK cells transfected with hyperdCas12a base editor and NFKB-driven crGFP*, 3 days post-transfection and stimulation with TNFα. To go with Fig. 1g.
