Extended Data Fig. 6: Radial phylogenetic tree of S1_15 showing the conservation of the galacto- recognition triad and N-acetyl-D-galactosamine specificity features.
From: Sulfated glycan recognition by carbohydrate sulfatases of the human gut microbiota
The tree comprises a total of 1906 sequences of which 1424 are Bacteroidetes; 172 are Planctomycetes; 119 are Kiritimatiellaeota; 57 are Proteobacteria; 53 are Verrucomicrobia. The annotations next to the colour code concern the presence or absence of conservation of the BT16246S-Gal/GalNAc (acc-code Q8A7A1) indicated residues and in this order: I100, D170, R171, H220, K461 and A462. These residues are crucial in substrate recognition and D170, R171, and H220 represent the galacto-recognition triad within S1_15 subfamily. For simplification the residue numbers have been omitted. For example, an I in black means an equivalent isoleucine is present; a grey and bold letter at any position means that the corresponding residue is replaced by that amino acid; a grey and italic letter at any position means that the equivalent position can be replaced by any type of amino acid; a bold grey letter followed by one-letter codes in parentheses indicates that the equivalent position is substituted by any of those amino acids. Branches having the same colour have the corresponding pattern in common. For clarity all labels and sequence accession codes have been omitted. Red filled circles designate sequences of S1_15 sulfatases from B. thetaiotaomicron (See Supplementary Fig. 6 for full tree).
