Extended Data Fig. 7: Conservation of the N-acetyl-D-galactosamine specificity features (Y463/W464) in S1_15 enzymes within PULs targeting chondroitin sulfate.
From: Sulfated glycan recognition by carbohydrate sulfatases of the human gut microbiota
Schematic representation of PULs targeting chondroitin sulfate aligned by orthologues of BT33336S-GalNAc. Light green background shows orthologues with Y463/W464, a dark green background highlights orthologues with F463/W464, a light blue background highlights orthologues with H463/W464, and a purple background highlights orthologues with Q463/W464. The numbering used corresponds to the sequence of BT33336S-GalNAc. A red background highlights the presence of GH88 and S1_27 (an endo 4S-chrondroitin sulfatase), which is encoded by a discrete genetic region not always physically localised next to the core PUL. A black background highlights a core block observed in CS PULs containing BT33336S-GalNAc orthologues. HP (protein of unknown function), S1 (sulfatase S1 with the respective subfamily number superscript), GHXX (glycoside hydrolase with X representing the family number), PL (polysaccharide lyase), DUF (domain of unknown function), HTCS (hybrid two-component system), SusC (starch utilization system C-like), SusD (starch utilization system D-like), SGBP (surface glycan binding protein).
