Extended Data Fig. 10: Conservation of the N-sulfate targeting features, W273/R290, in S1_11 enzymes within PULs targeting heparan sulfate.
From: Sulfated glycan recognition by carbohydrate sulfatases of the human gut microbiota
PULs targeting heparan sulfate (HS) aligned by orthologues of BT46566S-GlcNAc/GlcNS. Orthologues of BT46566S-GlcNAc/GlcNS with W273/R290 and W273/Q290 are highlighted with a green and blue background, respectively. A black background highlights a core block observed in HS PULs containing BT46566S-GlcNAc/GlcNS orthologues. HP (protein of unknown function), S1 (sulfatase S1 with the respective subfamily number superscript), GHXX (glycoside hydrolase with X representing the family number), PL (polysaccharide lyase), DUF (domain of unknown function), HTCS (hybrid two-component system), SusC (starch utilization system C-like), SusD (starch utilization system D-like), SGBP (surface glycan binding protein), MFS (major facilitator superfamily), ROK (repressor, ORF, kinase superfamily).
