Extended Data Fig. 9
From: A fungal dioxygenase CcTet serves as a eukaryotic 6mA demethylase on duplex DNA
Dot Blot analysis of concentration-dependent 6 mA (a) and 5mC (b) demethylation on green alga (Chlamydomonas reinhardtii) genomic DNA catalyzed by CcTet or D337F mutant. Upper panels show the representative antibody (anti-5mC or 6 mA) dot blot for the purified Chlamydomonas reinhardtii genomic DNA; lower panels show the methylene blue staining to validate the equal loading amount of DNA.
