Extended Data Fig. 4: Lys-CO2 modification of RuBisCO large subunit identified by proteome wide LysCarComp-MS performed on Synechocystis sp., related to Fig. 4. | Nature Chemical Biology

Extended Data Fig. 4: Lys-CO2 modification of RuBisCO large subunit identified by proteome wide LysCarComp-MS performed on Synechocystis sp., related to Fig. 4.

From: Chemoproteomic identification of CO2-dependent lysine carboxylation in proteins

Extended Data Fig. 4

a, LC-MS/MS spectra showing unambiguous evidence for hCit196 residue within TMTduplex-labelled RuBisCO large subunit peptide [TMT-GGLDFThCit(K196 + 43.01 Da)DDENINSQPFMR, MH + [Da] = 2452.16]. b, Close-up of TMTduplex reporter ions and immonium ions derived from (a). The mean RCO2 with SD from three independent experimental replicates is given.

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