Extended Data Fig. 6: CID661578-treated zebrafish in the basal state exhibit no changes in endocrine cell numbers.
From: MNK2 deficiency potentiates β-cell regeneration via translational regulation
a-c,g, Maximum projections confocal images of primary islets in Tg(ins:H2BGFP) larvae at 6 dpf after treatment with (a) DMSO, (b) CID661578 or (c) cercosporamide from 3-6 dpf. Quantification (g) of the total number of β-cells revealed no changes resulting from the treatments. Scale bar, 10 µm. n = 12 (Control), n = 11 (CID661578) and n = 11 (Cercosporamide). Data are presented as mean values ±SEM. d-f,h-i, Single-plane confocal images of primary islets in Tg(gcga:GFP);Tg(sst2:dsRed2) larvae at 6 dpf after treatment with (d) DMSO, (e) CID661578 or (f) cercosporamide from 3-6 dpf. Quantification of the total number of α-cells (h) and δ-cells (i) revealed no changes resulting from the treatments. Scale bar, 10 µm. For both (h and i), n = 14 (Control), n = 9 (CID661578) and n = 9 (Cercosporamide). Data are presented as mean values ±SEM. j, Glucose measurements in zebrafish larvae in the basal state treated with DMSO, CID661578 or cercosporamide for 1 day (3-4 dpf). Four larvae were pooled for each replicate. n = 5 (Control), n = 4 (CID661578) and n = 5 (Cercosporamide). One-way ANOVA test followed by Dunnett’s multiple comparisons test was used to assess significance for (j) ****P < 0.0001 (Control vs CID661578); *P = 0.0182 (Control vs Cercosporamide).
