Extended Data Fig. 9: CID661578.6 does not potently affect the activity of any kinase in an in vitro screen; and MNK2 expression in the pig pancreas.
From: MNK2 deficiency potentiates β-cell regeneration via translational regulation
a,b, An in vitro screen to assess the specificity of 1 µM CID661578.6 and 1 µM cercosporamide against a panel of 140 kinases. CID661578.6 did not alter the activity of any kinase by more than 50% (a), while cercosporamide inhibited the activity of several kinases, including MNK1 and MNK2 (b). Red arrows point to MNK1 and MNK2. c-e, Images of neonatal (c,d) and adult (e) pig pancreatic sections immunostained for MNK2, with insulin used as a marker of β-cells and CK7 as a marker of ductal cells. Scale bar, 50 µm. Similar staining results were obtained in sections from multiple pig pancreata.
