Extended Data Fig. 2: Enrichment of individual genotypes throughout full Evolink experiment.
From: Three-dimensional structure-guided evolution of a ribosome with tethered subunits
a–c, Positively enriched genotypes (purple) and negative enriched genotypes (dark gray) can be tracked throughout multiple time points throughout selection. Genotypes that drop out during selection can also be identified (light gray). Corresponding heat maps that reveal trends in selected tether lengths also helped inform designs. Generally, across the three libraries tested in this work, (a) the Broad Sampling Library, (b) the Designed Junction Library, and (c) the Designed Junction + Length Refined Library, log2-fold enrichment values between -6 to 6 are observed. Enrichment and heatmap data representative of three independent experiments.
