Extended Data Fig. 4: Cryo-EM processing of the Dot1L-H3K79Nle complex.
From: H2B Lys34 Ubiquitination Induces Nucleosome Distortion to Stimulate Dot1L Activity
The data was processed in RELION 3.1 softaware, the local resolution of the two final maps was estimated in Resmap software as colored in red to bule from 2.5 Å to 5.0 Å. The globular resolution of Dot1L-H3K79Nleactive was at 3.06 Å according to FSC (0.143) criterion, and the globular resolution of Dot1L-H3K79Nleinactive was at 3.27 Å. The Dot1L in Dot1L-H3K79Nleinactive complex was relatively flexible and lower resolution compared to nucleosome core parts, and it that can be further classified into three different Dot1L orientations (3.90 Å, 4.53 Å and 4.39 Å).
