Extended Data Fig. 7: Extended plots for the fluorescence-quenching assay for labelled apCC-Di, apCC-Di-AB containing additional controls.
From: De novo designed peptides for cellular delivery and subcellular localisation
(left) Fluorescence quenching assay for homomeric peptides. Controls include the parallel coiled coil CC-Di and swapping the fluorophore and the fluorescence quencher between the termini of apCC-Di. (right) Fluorescence quenching assay for the designed heteromeric peptides. The control peptides include labelling both apCC-Di-A and apCC-Di-B near the C-termini, which does not lead to quenching in an antiparallel orientation. Key: n and c indicate mutations near the N and C termini, respectively; 4CF, 4-Cyano-L-phenylalanine fluorophore; MSE, L-selenomethionine fluorescence quencher. Conditions: 100 μM concentration of each peptide, 50 mM sodium phosphate, pH 7.
