Extended Data Fig. 8: Extended bimolecular fluorescence complementation (BiFC) assay containing controls for homomerisation of apCC-Di-A and apCC-Di-B, and controls for heteromerisation of CC-Di-A and CC-Di-B (a parallel heteromeric system).
From: De novo designed peptides for cellular delivery and subcellular localisation
BiFC assay for apCC-Di and apCC-Di-AB. V1 and V2 are N- and C-terminal fragments of the Venus yellow fluorescent protein, where V1 represents the V1 mutant. Peptide names that precede the Venus fragment name denote fusion to the C termini of the coiled coil, for example apCC-Di-B-V2. Values are normalised according to cell density (OD600) and are presented as mean values±1 SD from n ≥ 3 technical replicate measurements (dots).
