Extended Data Fig. 9: Cell penetration of apCC-Di-B occurs at 4 °C and in the presence of endocytosis inhibitors.

a, Representative confocal images of HeLa cells treated for 1 h with 2 µM TAMRA-labelled apCC-Di-B at 37 ^oC and 4 °C. These representative images are taken from n = 3 biological replicates. b, The same for cells at 37 °C pre-incubated with 0.1% DMSO (as a control) and with endocytosis inhibitors 30 µM MiTMAB, 80 µM Dynasore, and 0.1% NaN₃ in the DMEM before treatment with 2 µM TAMRA labelled apCC-Di-B peptide in media containing each of the inhibitors for 1 h. apCC-Di-B remains cell penetrating at 4 °C and in the presence of the inhibitors and at 37 °C, as shown by TAMRA fluorescence in the cytoplasm and nucleus. Scale bar 10 µm. These representative images are taken from n = 3 biological replicates.