Extended Data Fig. 6: Cartoon representation of mutation positions in designed coiled-coil (CC) dimers for the fluorescence quenching assay.
From: De novo designed peptides for cellular delivery and subcellular localisation
For homomeric CCs, the fluorophore was incorporated near the N termini and a fluorescence quencher near the C termini. For parallel CCs (top left) this should lead to fluorescence, whereas for antiparallel CCs (top right) this should lead to quenching. For antiparallel heteromeric CCs, incorporating the fluorophore and fluorescence quencher near the same termini (bottom left) should lead to fluorescence, whereas incorporating at opposite termini should lead to quenching (bottom right).
