Extended Data Fig. 3: Identification of the base-editing window of TaRGET-ABE-C2 system.
From: Hypercompact adenine base editors based on a Cas12f variant guided by engineered RNA
The substitution profile of TaRGET-ABE-C2 was explored for five endogenous sites in HEK293T cells to define a base editing window. The endogenous sites were selected from the validated sites showing substantial A-to-G conversion activities and also carrying multiple adenine sequences in the PAM-proximal region. The TaRGET-ABE system elicits A-to-G conversions in the range of positions 2–6, but most predominantly at the positions 3-4. The intensity of colors is not proportional to the editing efficiency, but highlights the qualitatively high efficiency positions.
