Extended Data Fig. 7: MDH1 S189 glycosylation promotes PDAC cell proliferation.
From: O-GlcNAcylation promotes pancreatic tumor growth by regulating malate dehydrogenase 1
a, Immunoblotting of MDH1 expression in HPDE and PDAC cells upon stably transfection with scramble or shMDH1. b, Relative cell proliferation rate of HPDE and PDAC cells upon stably transfection with scramble or shMDH1 (n = 3 independent assays). c, Immunoblotting of MDH1 in PANC-1 cells stably expressing scramble shRNA, shMDH1, shRNA-resistant WT or S189A MDH1. d and e, Cell proliferation rate of SW1990 (c) and PANC-1 cells (d) stably expressing scramble shRNA, shMDH1, shRNA-resistant WT or S189A MDH1 (n = 5 independent assays). f and g, Relative clone numbers generated from SW1990 (f) and PANC-1 cells (g) stably expressing scramble shRNA, shMDH1, shRNA-resistant WT or S189A MDH1 (n = 5 independent assays). h and i, Cell proliferation rate of SW1990 (h) and PANC-1 cells (i) reconstituted with WT or S189A MDH1 upon OGT overexpression (n = 5 independent assays). Immunoblots are representative of three independent experiments. Error bars of data in b, d, e, f, g, h and i denote the means ± SD. Statistical analyses were performed by two-tailed Student’s t-test.
