Extended Data Fig. 2: Kras activated mutation promotes OGT expression in PDAC cells.
From: O-GlcNAcylation promotes pancreatic tumor growth by regulating malate dehydrogenase 1
a, Immunoblotting of GFPT, OGT and O-GlcNAc levels in HPDE cells upon ectopic expression of KrasG12D. b, Immunoblotting of O-GlcNAc levels in SW1990 cells upon depletion of Kras. c, Immunoblotting of O-GlcNAc levels upon OGT depletion in HPDE cells expressing KrasG12D. d, Cell proliferation rate of HPDE cells expressing KrasG12D upon OGT depletion (n = 3 independent assays). Immunoblots are representative of three independent experiments. Error bars of data in d denote the means ± SD. Statistical analyses were performed by two-tailed Student’s t-test.
