Extended Data Fig. 5: Immunogenicity, hematology and histological evaluation of LNP-CasRx-pre-gCtsl treatment in C57BL/6 mice.
From: Cas13d knockdown of lung protease Ctsl prevents and treats SARS-CoV-2 infection
a, Cytokine and chemokine transcript levels in mouse lung were unchanged by LNP-CasRx-pre-gCtsl treatment. C57BL/6 mice lung issues were collected at 72 h following the treatments. All transcript levels are normalized to Gapdh. Data are presented as mean ± SEM for each group (n = 4, 2 females and 2 males). P values were calculated by two-tailed Mann-Whitney U test, NS, not significant. b, Hematology was not impaired by LNP-CasRx-pre-Ctsl treatment. WBC, white blood cells; PLT, platelets; RBC, red blood cells; HGB, hemoglobin. Data are presented as box and whisker plots of each group with n = 8 (4 females and 4 males in each group except for 3 females and 5 males in LNP-CasRx-pre-gControl group). Whiskers are min to max. P values were calculated by two-tailed Mann-Whitney U test, NS, not significant. c, No substantial histopathological changes were produced by LNP-CasRx-pre-gCtsl treatment in indicated organ tissues. Two sections of each organ from 3 mice per group were evaluated after H&E staining and the representative images are presented.
