Extended Data Fig. 7: CasRx-mediated Ctsl knockdown reduces viral burden in the lungs of K18-hACE2 mice on Day 4 after SARS-CoV-2 infection.
From: Cas13d knockdown of lung protease Ctsl prevents and treats SARS-CoV-2 infection
The experiment design was same as in Fig. 5a. a, Viral N gene transcript level. b, Viral E gene transcript level. c, Western blot of viral N protein and Ctsl in mouse lung. All mice were subjected to this analysis. Each lane represents an individual mouse. Calnexin was used as a loading control. The ratio of N protein or Ctsl to calnexin signal is listed under the blot. d, Integrated density of N protein (upper panel) and Ctsl (lower panel) over the loading control. e, Representative images for N protein immunostaining in the lungs. One section per mouse was subjected to IHC analysis and only one representative image from each group was presented. f, IHC score for N protein. g, Ctsl transcript levels. a, b, g, All transcript levels are normalized to Gapdh. P values were calculated by one-tailed Mann-Whitney U test, grand mean. * P < 0.05, ** P < 0.01, *** P < 0.001.
