Extended Data Fig. 3: Characterization of empty LNPs (eLNPs) and LNPs encapsulating luciferase mRNA.
From: Cas13d knockdown of lung protease Ctsl prevents and treats SARS-CoV-2 infection
a, Size distribution. The size of two batches of eLNPs and LNP-Luc were analyzed by Zetasizer. b, Representative transmission electron microscopic images. The experiment was repeated twice with similar results. c, Zeta potential. eLNPs and LNP-Luc were diluted to 0.8 μg/μl with 1X DPBS for measurement. Data are presented as mean ± SD of two batches of samples. d, Encapsulation rate and stability of LNP-Luc. The encapsulation efficiency of LNP-Luc was determined by RiboGreen assay on days 0, 2, 4, 6, 8 and 10 following its storage at 4°C.
