Fig. 1: The concept of spatiotemporal control over NTP-driven reactions via LAGOON.
From: Spatiotemporally controlled generation of NTPs for single-molecule studies
a, Schematic of a LAGOON experiment monitoring the helicase-catalyzed unwinding of DNA as an example, depicting FOVs after (FOV 1), during (FOV 2) and before (FOV 3) ATP uncaging. Y-shaped DNA constructs with Cy3 (green) and Cy5 (red) fluorophores attached at the beginning of the duplex region are immobilized on the surface via streptavidin–biotin interactions with biotinylated PEG. Unwinding of the DNA duplex by the T4 helicase results in the decrease of the initially high FRET. b, Structure and uncaging of DMNPE-caged ATP.
