Extended Data Fig. 4: Characterization of remodeling intermediates observed with LAGOON in the single-turnover regime. | Nature Chemical Biology

Extended Data Fig. 4: Characterization of remodeling intermediates observed with LAGOON in the single-turnover regime.

From: Spatiotemporally controlled generation of NTPs for single-molecule studies

Extended Data Fig. 4

Histograms of four consecutive FRET plateaus (left) and corresponding FRET changes (right) in time traces (N = 209) showing the remodeling of individual nucleosomes by Chd1 in a LAGOON experiment with pulsed uncaging in the presence of 10 mM DMNPE-ATP. Pulse duration 90 ms, pulse period 8 s, uncaging power density 100 W/cm2.

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