Extended Data Fig. 7: Cyclophilin binding profiles using fluorescence polarization of fluorescein-labeled macrocycles.
From: Discovery and molecular basis of subtype-selective cyclophilin inhibitors

Each cyclophilin was titrated against 0.5 nM fluorescein-labeled macrocycle a, A26-Fl; b, B52-Fl; or c, B53-Fl. Trends for selectivity follow those observed in the prolyl-isomerase assay. Cyclophilins in the legend below the dashed line are either prolyl-isomerase inactive or require much higher concentrations to observe prolyl isomerization in vitro. Kd values and error bars reflect mean ± s.e.m. of three technical replicates. Data points and error bars reflect mean ± s.d. of individual assays at one dose.