Extended Data Fig. 8: Quantification of mitochondrial localization in HeLa cells by fluorescence microscopy of Cy5-conjugated compounds. | Nature Chemical Biology

Extended Data Fig. 8: Quantification of mitochondrial localization in HeLa cells by fluorescence microscopy of Cy5-conjugated compounds.

From: Discovery and molecular basis of subtype-selective cyclophilin inhibitors

Extended Data Fig. 8

HeLa cells were treated with Cy5-conjugated compounds and analyzed for a, total identifiable Cy5 spots; b, Cy5 spots per well; c, mean fluorescence intensity of identified Cy5 spots; d, sum of fluorescence intensity of identified Cy5 spots; e, mean Cy5 fluorescence intensity per cell; f, sum of Cy5 fluorescence in all measured cells; g, percent of Cy5 spots that overlap >70% with Mitotracker Green co-stain; and h, fluorescence intensity of Cy5 spots that overlap >70% with Mitotracker Green. i, values of data shown in a-h. Values and error bars reflect mean ± s.d. of three technical replicates.

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