Extended Data Fig. 3: Optimizing reporter length for enhanced collateral activity.
From: Engineered LwaCas13a with enhanced collateral activity for nucleic acid detection
a and b, Representative gel images of the cleavage of 5′-FAM-labeled U5, U11, U15, and U20 reporters by WT LwaCas13a, RBD #3L, and RBD#4L over 0–30 min. RBD fusions and WT were tested side-by-side. c–e, Quantified percentage of cleaved products as shown in a and b, represented by mean ± s.e.m. of three technical replicates from two independent experiments. Gels were analyzed by ImageJ. The percentage of cleaved products was calculated by the intensity of product bands divided by the summed intensity of the product and substrate bands within one lane and normalized to the reporter-only control.
