Extended Data Fig. 7: Structure determination of mTRPV2E2_2-APB.
From: Structural mechanisms of TRPV2 modulation by endogenous and exogenous ligands
a, Representative cryo-EM micrograph of mTRPV2E2_2-APB. n = 3 independent experiments. b, Flowchart of image processing for mTRPV2E2_2-APB particles. c, The density map of mTRPV2E2_2-APB colored by local resolution. d, Distribution of orientations over azimuth and elevation angles for particles included in the calculation of the final map. e, Gold-standard Fourier Shell Correlation (FSC) curves calculated with different masks in cryoSPARC. f, The FSC curve for cross-validation between the map and the model of mTRPV2E2_2-APB. g, Sample maps of six transmembrane helices (TMs) in mTRPV2E2_2-APB. The density corresponding to 2-APB is also shown. The density is shown as a mask around each TM at the level of 0.006 in UCSF Chimera. The maps are low-pass filtered to 3.27 Å and sharpened with a B factor of -99.6 Å2.
