Extended Data Fig. 1: Structure determination of mTRPV2CHL.
From: Structural mechanisms of TRPV2 modulation by endogenous and exogenous ligands
a, Size-exclusion chromatography of mTRPV2CHL on Superose 6 (GE Healthcare) and SDS-PAGE analysis of the final sample. n = 3 independent experiments. b, Representative cryo-EM micrograph of mTRPV2CHL. n = 3 independent experiments. c, Flowchart of image processing for mTRPV2CHL particles. d, Angular distribution plot of particles included in the final C4-symmetric 3D reconstruction of mTRPV2CHL. e, The density map of mTRPV2CHL colored by local resolution (Å). f, The Gold standard Fourier Shell Correlation (FSC) curve of the final 3D reconstruction of mTRPV2CHL, and the FSC curve for cross-validation between the map and the model of mTRPV2CHL. g, Sample maps of six transmembrane helices (TMs) in mTRPV2CHL. The density corresponding to cholesterol is also shown. The density is shown as a mask around each TM at the level of 0.016 in UCSF Chimera. The maps are low-pass filtered to 3.17 Å and sharpened with a B factor of –99 Å2. h, Lipid densities in mTRPV2CHL map is colored in yellow. i, Comparison of the atomic models of mTRPV2CHL and ratTRPV1 (7MZ6).
