Fig. 2: S0-generating/degrading enzymes modulate LPO and ferroptosis.
From: Hydropersulfides inhibit lipid peroxidation and ferroptosis by scavenging radicals
a, Influence of depleting CSE (siCSE) on the loss membrane integrity triggered by treatment of HeLa cells with either RSL3 (5 µM) (left two panels) or CHP (40 µM) (right two panels), as measured by the CellTox Green cytotoxicity assay. n = 3. b, Influence of overexpressing CSE (CSE OE) on the loss of membrane integrity triggered by treatment of HeLa cells with either RSL3 (5 µM) (left two panels) or CHP (40 µM) (right two panels), as measured by the CellTox Green cytotoxicity assay. n = 3. c, Influence of depleting CSE (siCSE) or ETHE1 (siETHE1) on the loss membrane integrity triggered by treatment of HeLa cells with RSL3 (5 µM), as measured by the CellTox Green cytotoxicity assay. Fluorescence is normalized to the lysis control (lethal fraction). n = 3. d, Influence of depleting CSE (siCSE) on the oxidation of BODIPY-C11, as induced by incubation with RSL3 (5 µM) for 4 hours. n = 3. P = 0.0049. e, Influence of depleting ETHE1 (siETHE1) on the oxidation of BODIPY-C11, as induced by incubation with RSL3 (2 µM) for 3 hours. n = 3. P = 0.0236. Data are presented as mean values. For CellTox assays, error bars represent s.e.m. For the rest, error bars represent s.d. * P ≤ 0.05 and ** P ≤ 0.01 based on a two-tailed unpaired t-test.
