Extended Data Fig. 6: Protein S-nitrosylation of ACE2 by NMT5.
From: Targeted protein S-nitrosylation of ACE2 inhibits SARS-CoV-2 infection
a, HeLa-ACE2 cells were treated with 10 μM NMT5, NMT6, or NMT8. After 1 h, cell lysates were subjected to biotin-switch assay for protein S-nitrosylation, detected by immunoblotting with anti-ACE2 antibody. The ascorbate minus (Asc-) sample served as a negative control. b, Ratio of SNO-ACE2/input ACE2 protein. Data are mean + s.e.m. by one-way ANOVA with Tukey’s multiple comparisons. n = 3 biological replicates.
