Extended Data Fig. 9: Targeted S-nitrosylation of ACE2 by NMT5 in the presence of envelope (E) viroporin protein.
From: Targeted protein S-nitrosylation of ACE2 inhibits SARS-CoV-2 infection
Kinetic analysis of S-nitrosylation of ACE2. E protein plasmid was transiently transfected into HEK293 cells stably-expressing Spike protein cells. Subsequently, the cells were harvested and plated onto ACE2-expressing HeLA cells in the presence or absence of 20 µM NMT5. At the indicated timepoints, cells were subjected to biotin-switch assay. Ordinate shows ratio of SNO-ACE2/total input ACE2 protein above the baseline value, defined as 1.0. Data are mean + s.e.m. by two-way ANOVA with Fisher’s LSD test. n = 5 biological replicates.
