Extended Data Fig. 1: S-Nitrosylation of ACE2 persists for at least 12 hours.
From: Targeted protein S-nitrosylation of ACE2 inhibits SARS-CoV-2 infection
a, HeLa-ACE2 cells were exposed to 100 μM SNOC; 30 min later the cells were incubated in serum free medium for the time periods indicated. Cell lysates were then subjected to biotin-switch assay to assess protein S-nitrosylation, which was detected by immunoblotting with anti-ACE2 antibody. b, Ratio of SNO-ACE2/input ACE2 protein. Data are mean + s.e.m. by one-way ANOVA with Fisher’s LSD multiple comparisons. n = 3 biological replicates.
