Extended Data Fig. 7: Cryo-EM data processing of hTRPV3-G573S.
a, Flow chart of cryo-EM data processing. A total of 691,221 particles were picked from 2,594 micrographs. A representative motion-corrected micrograph of the dataset is shown here (Bar = 400 Å). Several rounds of 2D and 3D classifications were conducted to clean particles, followed by Bayesian Polish and CTF Refine to improve image quality. The final map was refined at 3.64 Å and 2.93 Å for hTRPV3-G573S-C4 and hTRPV3-G573S-C2 according to the GSFSC criterion, respectively. (b & e) Local resolution distribution of the sharpened map of the hTRPV3-G573S-C4Trpvicin and hTRPV3-G573S-C2Trpvicin. (c & f) Particle angular distribution calculated in cryoSPARC for the final reconstruction of hTRPV3-G573S-C4Trpvicin and hTRPV3-G573S-C2Trpvicin. (d & g) Fourier Shell Correlations (FSC) of the final map of the hTRPV3-G573S-C4Trpvicin and hTRPV3-G573S-C2Trpvicin, calculated between two independently refined half-maps before (blue) and after (red) post-processing, overlaid with an FSC curve calculated between the cryo-EM density map and the structural model shown in black.
