Extended Data Fig. 8: Condensation of SEU is evolutionarily conserved.
From: Condensation of SEUSS promotes hyperosmotic stress tolerance in Arabidopsis
a, The phylogenetic tree of SEU homologs inferred using the Neighbor-Joining method. Evolutionary analysis was performed in MEGA X. b, Multiple sequence alignment of SEU homologs. The regions corresponding to IDR1, LDB and IDR2 were indicated. c, Zoom-in of the region marked by a rectangle in (b) showing the conservation of the two predicted α-helical peptides between SEU homologs. d, The structures of SEU homologs as predicted by AlphaFold. e, Representative confocal microscopic images of tobacco epidermal cells expressing SEU homologs. The cells were treated with or without NaCl for 5 min. Scale bars, 5 μm. f, The phylogenetic tree of AtSEU and its counterparts in yeast inferred using the Neighbor-Joining method. g, Top, protein domain structure of fission yeast Ssn6. Bottom, prediction of the disordered regions by IUpred algorithm. h, Representative confocal microscopic images of yeast cells expressing Ssn6. The cells were treated with or without sorbitol for 5 min. Scale bars, 2 μm.
