Extended Data Fig. 1: Expanded set of timepoints for substrate competition assay.
From: Designer installation of a substrate recruitment domain to tailor enzyme specificity
Expanded set of timepoints for substrate competition assay for target peptide (red) and off-target (light blue) at 1 (a), 10 (b), and 100 (c) μM peptide substrate (2, 20, and 200 μM total peptide respectively). Raw data points are represented by black dots superimposed on plots. Data bars present mean values +/− SEM across three distinct reactions (n = 3, frequently hidden under data points). Error bars are centered on the mean. (d) Exact timepoints collected for 1, 10, and 100 μM. Timepoint 2 is displayed in Fig. 4 in main text and t1 at 100 μM is used in competition reaction mathematical modeling.
