Extended Data Fig. 5: Cryo-EM image processing for the PSMD2:Fab dataset.
From: Targeted degradation via direct 26S proteasome recruitment
a, Representative micrograph. b, Representative 2D class averages. c, Image processing flowchart. Blue-red color scaling indicates estimated local resolution. d, Particle angular (theta/phi) orientation diagram (cisTEM). Scale at right indicates number of particles per angular bin. e, Masked Fourier shell correlation (FSC) plots for final masked refinement. Gray horizontal line indicates FSC = 0.143. Vertical line with arrow indicates refinement limit for the final refinement iterations.
