Extended Data Fig. 9: Aberrant WEE1 kinase activity induces cell apoptosis.
From: SIRT1 deacetylates WEE1 and sensitizes cancer cells to WEE1 inhibition
(a) A U2OS cell line to express siRNA-resistant SFB-WEE1 under the control of a tetracycline-inducible promoter was generated. The resulting cells were transfected with the indicated siRNAs and then treated with doxycycline (1 μg/mL) to induce the expression of WEE1. 48 hr later, percentages of apoptotic cells were determined by FACS analysis. Results shown are means of three independent experiments and were presented as means ± SEM. P-values were determined by two-tailed Student’s t-test. (b) HEK293T cells were transfected with the indicated plasmids for 24 hr. Cells were then fixed, permeabilized, and stained with anti-Flag antibody. Scale bar: 10 μm. Data in B were independently replicated at least three times, with similar results.
