Extended Data Fig. 6: FINO2-0 and FINO2-2 accumulate in the ER, and FINO2-1/FINO2-3/FINO2-4 do not accumulate in the plasma membrane.
From: Identification of essential sites of lipid peroxidation in ferroptosis
a. Structure of FINO2-0. b. Dose-response curve of HT-1080 cells treated with FINO2-0 ± fer-1. Data are represented as mean ± SEM, n=3. c. Confocal fluorescence images of HT-1080 cells treated for 3 hours with 3 µM FINO2-1 alone or with 3 µM fer-1. d. Confocal fluorescence image of HT-1080 cells treated with 3 µM FINO2-0. f. Confocal fluorescence imaging of HT-1080 cells treated with FINO2-1 (3 µM) and fer-1 (3 µM) for 3 hours, co-stained with BODIPY TR ceramide. g. Structure of FINO2-2. h. Dose-response curve of HT-1080 cells treated with FINO2-2 ± fer-1. Data are represented as mean ± SEM, n=3. i. SRS and fluorescence imaging of HT-1080 cells treated with 20 µM FINO2-2 and 2 µM fer-1, and stained with Nile Red and Lysotracker green. j. Confocal fluorescence image of HT-1080 cells treated with 3 µM FINO2-1 and 3 µM fer-1, and costained with CellMask Deep Red. k. Confocal fluorescence image of HT-1080 cells treated with 3 µM FINO2-3 or 3 µM FINO2-4, and 3 µM fer-1, and co-stained with CellMask Deep Red.
