Extended Data Fig. 2: Knockdown of PUFA-related genes shows some impact on D-PUFA potency, but no observable decrease in incorporation.
From: Identification of essential sites of lipid peroxidation in ferroptosis
a. Dose-response curves of stable non-targeting (NT) or ACSL knockdown HT-1080 cells pretreated with EtOH or D-PUFAs and then treated with RSL3. Data are represented as mean ± SEM, n=3. b. Dose-response curves of stable nontargeting (NT) or AGPAT3 knockdown HT-1080 cells pretreated with EtOH or D-PUFAs and then treated with RSL3 or IKE. Data are represented as mean ± SEM, n=3. c. qPCR data of stable shRNA knockdowns. Data are represented as mean of three technical replicates ± upper and lower limit of 95% confidence interval. d. SRS images of shNT, shACSL5, and shAGPAT3 HT-1080 cells treated with DHA-d10 (10 µM) (left), and quantification of their signal intensity (right), Data are plotted as mean ± SEM, n=3.
