Extended Data Fig. 1: Amino acid resolution nucleosome affinity proteomics screen.

a, Schematic of nucleosome affinity proteomics screen. b, Coomassie-stained SDS-polyacrylamide denaturing gel (top) and ethidium bromide-stained native gel (bottom) of biotinylated nucleosome library. These experiments were repeated independently two times with similar results. c, Triplicate pulldowns run briefly on SDS polyacrylamide gels and stained with Coomassie blue prior to excision and in-gel trypsin digestion. Unused replicates of wild-type nucleosome pulldowns marked with an asterisk. d, Western blots following pulldowns from HEK293T nuclear lysates using indicated biotinylated nucleosomes. These experiments were performed once. WT, wild-type.