Extended Data Fig. 3: KDM2A/B-nucleosome complex purification and 2D classification. | Nature Chemical Biology

Extended Data Fig. 3: KDM2A/B-nucleosome complex purification and 2D classification.

From: Structural basis of paralog-specific KDM2A/B nucleosome recognition

Extended Data Fig. 3

a, Chromatograms displaying normalized 280 nm absorbance from size exclusion purification of KDM2B/WT nucleosome (left), KDM2B/H3K36C-UNC8015 nucleosome (middle), and KDM2A/H3K36C-UNC8015 nucleosome (right) complex reconstitutions. These experiments were performed once. b, Highest populated 2D classes attained from cryo-EM analysis of indicated samples, with selected top views of KDM2A/B-unbound and bound nucleosomes.

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