Fig. 2: Peptide exchange kinetics of the CLIP peptide for the investigated DRB1 natural variants.
From: MHC-II dynamics are maintained in HLA-DR allotypes to ensure catalyzed peptide exchange
a, Representative plot of the CLIP-FITC peptide dissociation shown for DRB1*01:01, as detected by a change in fluorescence polarisation (ΔFP). b, Dissociation rates (koff) of the CLIP-FITC peptide measured for 12 DRB1 natural variants in competition experiments in the presence and absence of DM. c, DM susceptibility calculated from dissociation experiments as described in Methods. The dashed line represents the average value of DM susceptibility (0.24 µM−1 min−1), which separates the allotypes into two groups of high or low DM susceptibility. Data in b and c are presented as mean ± s.d. (n = 3 independent experiments). d, Representative plot of the CLIP-FITC peptide association shown for DRB1*01:01. e, Heat map of the association initial velocities (kon) of the CLIP-FITC peptide measured for 12 DRB1 natural variants in the presence of different DM concentrations. The data result from at least two independent experiments with at least three replicates in one experiment.
