Extended Data Fig. 1: Chemical and enzymatic sequencing methods for resolving DNA modifications.
From: Direct enzymatic sequencing of 5-methylcytosine at single-base resolution
a) Methods differ in their use of protection or modification steps to alter C, 5mC or 5hmC. They differ in deamination steps with chemical or enzymatic reagents. In each method, C, 5mC, or 5hmC are detected based on the pattern of C-to-T changes in sequencing, resulting in different possible bases that can be confounded with 5mC. b) Shown are the anticipated sequencing results for C, 5mC and 5hmC in CpG versus CpH contexts.
