Extended Data Fig. 1: SEC and negative staining TEM demonstrate formation of complexes between Prc K477A and apo-rNDM-1.
From: In-cell kinetic stability is an essential trait in metallo-β-lactamase evolution
a) SDS-PAGE analysis of SEC fractions corresponding to samples containing Prc K477A and either holo-rNDM-1 (top) or apo-rNDM-1 (bottom). b) Negative staining TEM of Prc K477A (top) or complexes of Prc K477A and apo NDM-1 (bottom). Samples for TEM analysis were obtained from SEC of the corresponding mixtures (shown in Fig. 4a), at the elution volume (VE in mL) indicated. Panels a, b show one of two independent experiments.
