Extended Data Fig. 5: Complementation with catalytically inactive BT4193 does not rescue loss of DPP4 activity in B. thetaiotaomicron lysate.
From: Chemoproteomic identification of a DPP4 homolog in Bacteroides thetaiotaomicron
Quantification of initial velocities of fluorogenic peptide substrate cleavage (ex/em: 380/460 nm for AMC substrates; ex/em: 355/460 nm for ACC substrates) in lysate generated from wild type (WT), knockout, and complemented B. thetaiotaomicron strains. Data represent the mean ± SEM of 8 independent replicates for WT and ∆BT4193 and 9 independent replicates for ∆BT3254, ∆BT4193 ∆BT3254, ∆BT4193::BT4193WT, and ∆BT4193::BT4193S606A. Statistical significance was determined using a one-way ANOVA test with post hoc Dunnett’s multiple comparisons tests compared with wild type (GP-AMC: p < 0.0001; WT-∆BT4193, p < 0.0001; WT-∆BT3254, p = 0.55; WT-∆BT4193 ∆BT3254, p < 0.0001; WT-∆BT4193::BT4193WT, p = 0.0004; WT-∆BT4193::BT4193S606A, p < 0.0001; AP-ACC: p < 0.0001; WT-∆BT4193, p < 0.0001; WT-∆BT3254, p = 0.96; WT-∆BT4193 ∆BT3254, p < 0.0001; WT-∆BT4193::BT4193WT, p < 0.0001; WT-∆BT4193::BT4193S606A, p < 0.0001; AAP-ACC: p < 0.0001; WT-∆BT4193, p = 1.00; WT-∆BT3254, p < 0.0001; WT-∆BT4193 ∆BT3254, p < 0.0001; WT-∆BT4193::BT4193WT, p = 1.00; WT-∆BT4193::BT4193S606A, p = 0.77; ***P < 0.001; ****P < 0.0001).
